A new, rapid in vivo method to evaluate allergic responses through distinctive distribution of a fluorescent-labeled immune complex: Potential to investigate anti-allergic effects of compounds administered either systemically or topically to the skin
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- 作者：Kouya Yamaki ; yam@kobepharma-u.ac.jp" class="auth_mail" title="E-mail the corresponding author ; Shin Yoshino
- 关键词：ARE ; average radiant efficiency ; ASDIS ; Anaphylaxis-dependent Spotted Distribution of a fluorescent-labeled Immune complex in Skin ; ELISA ; enzyme-linked immunosorbent assay ; FITC ; fluorescein isothiocyanate ; Ig ; immunoglobulin ; OVA ; ovalbumin ; PBS ; phosphate-buffered saline ; PE ; phycoerythrin ; PSA ; passive systemic anaphylaxis
- 刊名：Journal of Immunological Methods
- 出版年：2016
- 出版时间：January 2016
- 年：2016
- 卷：428
- 期：Complete
- 页码：58-68
- 全文大小：2585 K
文摘
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A new, rapid method to test IgE-dependent allergic responses in mice was established.
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An injection of immune complex induced its distinctive spotted distribution in skin.
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This phenomenon was induced and evaluated by in vivo imaging within 15 min.
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The phenomenon caused by IgE immune complex was anaphylaxis- and mast cell-dependent.
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This method has advantages in screening of anti-allergic drug and other formulations.