C2-substituted 8-aza-7-deaza-2′-deoxyadenosines as environmentally sensitive fluorescent nucleosides for discriminating apurinic/apyrimidinic sites in DNA duplex

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• 作者：Takumi Yamauchi ; Takuya Takeda ; Masaki Yanagi ; Noriki Takahashi ; Azusa Suzuki ; Yoshio Saito ; ^{saitoy@chem.ce.nihon-u.ac.jp}
• 关键词：Nucleoside ; DNA ; Fluorescent probe ; AP site
• 刊名：Tetrahedron Letters
• 出版年：2017
• 出版时间：11 January 2017
• 年：2017
• 卷：58
• 期：2
• 页码：117-120
• 全文大小：882 K
• 卷排序：58

文摘

Various environmentally sensitive fluorescent (ESF) nucleosides were synthesized. These are first ESF nucleosides possessing a C2-substituted azadeazaadenine skeleton. Compound 1c exhibited a remarkably high solvatochromicity (Δλ = 111 nm). 1c-containing DNA probes clearly discriminated an AP site in target DNA.