Induction of interleukin 2 expression in the liver for the treatment of H22 hepatoma in mice

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• 作者：Wei Cheng¹ ; Lanfang Miao¹ ; Haiying Zhang ; Ou Yang ; He Ge ; Yanru Li ; Lin Wang ; ^{wanglin@jlu.edu.cn}
• 关键词：RU486 regulatory system ; Cancer gene therapy ; Hydrodynamics-based procedure
• 刊名：Digestive and Liver Disease
• 出版年：2013
• 出版时间：January, 2013
• 年：2013
• 卷：45
• 期：1
• 页码：50-57
• 全文大小：1858 K

文摘

Background and aims

We designed this study to evaluate the ability of a plasmid carrying an RU486 regulatory system to induce expression of interleukin-2 (IL-2) gene and to examine the antitumour efficacy of the induced IL-2 gene.

Methods

The plasmid pRS-mIL-2,which contains an RU486 inducible system and IL-2 gene was injected into mice. Sera and tissues from liver, spleen, lungs and kidneys were taken to test the properties of the plasmid. To examine the antitumour efficacy of pRS-mIL-2, tumours were established in the liver by direct inoculation of H22 hepatoma cells.

Results

The IL-2 levels in serum correlated with the dose of plasmid and RU486. High and sustained IL-2 levels could be achieved by administration of RU486 every day. The mRNA of transgene IL-2 was found only in the liver. Treatment of mice with pRS-mIL-2 plus RU486 resulted in the significant reduction in tumour volume compared with control groups.

Conclusions

Tight temporal and spatial control of transgene IL-2 expression can be achieved by a plasmid containing an RU486 inducible system driven by liver specific promoter. pRS-mIL-2 exhibited strong antitumour efficacy following consecutive induction with RU486.