Development of a rapid on-site detection method for pork in processed meat products using real-time loop-mediated isothermal amplification
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- 作者：Shin-Young Lee1 ; Mi-Ju Kim1 ; Yeun Hong ; Hae-Yeong Kim ; hykim@khu.ac.kr" class="auth_mail" title="E-mail the corresponding author
- 关键词：Direct real-time LAMP ; Meat authentication ; Pork ; On-site detection
- 刊名：Food Control
- 出版年：2016
- 出版时间：August 2016
- 年：2016
- 卷：66
- 期：Complete
- 页码：53-61
- 全文大小：1947 K
文摘
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LAMP primers were designed based on the D-loop gene in pork and the 18S rRNA gene.
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The method was able to detect pork within 30 min without DNA extraction.
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Forty-two processed meat products were analyzed using this direct, real-time LAMP method.
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This assay can be applied to the on-site identification of pork in processed meat.