Structure and chain conformation of a (1 → 6)-α-d

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• 作者：Hengxiang Cui ; Qin Liu ; Yongzhen Tao ; Hongfeng Zhang ; Lina Zhang ; Kan Ding
• 关键词：Pueraria lobata ; (1 → ; 6)-α ; -d-Glucan ; Chain conformation ; Antioxidant effect ; Sulfated polysaccharide
• 刊名：Carbohydrate Polymers
• 出版年：2008
• 出版时间：21 November 2008
• 年：2008
• 卷：74
• 期：4
• 页码：771-778
• 全文大小：517 K

文摘

A water soluble glucan, PLB-2C, was isolated from the water extract of the root of Pueraria lobata (Willd) Ohwi using anion-exchange and gel permeation chromatography. Its structure was investigated by gas chromatography (GC), gas chromatography–mass spectrometry (GC–MS), infrared (IR) spectra, and nuclear magnetic resonance (NMR) spectroscopy of heteronuclear single quantum coherence (HSQC) and heteronuclear multiple bond correlation (HMBC) techniques. The results indicated that PLB-2C was a linear glucan composed of (1 → 6)-α-d-Glcp. Chain conformation study showed that the polysaccharide took random coil compact conformation. In vitro cell viability assay by MTT method, its sulfated derivative PLB-2CS which was substituted at 2-O, 3-O, 4-O positions, at 0.1, 1, and 5 mg/ml, could attenuate PC12 cell damage significantly caused by hydrogen peroxide.