Transcriptome analysis of normal and mantled developing oil palm flower and fruit

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• 作者：Jeremy R. Shearman^a ; ^{jeremy.she@biotec.or.th} ; Chatchawan Jantasuriyarat^b ; ^{fscicwj@ku.ac.th} ; Duangjai Sangsrakru^a ; ^{duangjai@biotec.or.th} ; Thippawan Yoocha^a ; ^{thippawan.yoo@biotec.or.th} ; Apichart Vannavichit^c ; ^{vanavichit@gmail.com} ; Somvong Tragoonrung^a ; ^{somvong.tra@biotec.or.th} ; Sithichoke Tangphatsornruang^a ; ^{sithichoke.tan@biotec.or.th}
• 关键词：DE ; differentially expressed ; GO ; gene ontology ; qPCR ; quantitative PCR ; RdDM ; RNA-directed DNA methylation pathway
• 刊名：Genomics
• 出版年：2013
• 出版时间：May, 2013
• 年：2013
• 卷：101
• 期：5
• 页码：306-312
• 全文大小：399 K

文摘

<em>Elaeis guineensisem> (oil palm) accounts for a large and increasing proportion of the world's cooking oil production. Cloning via somatic embryogenesis results in a somaclonal variant known as mantled which produce fruit with little to no oil yield. The mantled phenotype is believed to be epigenetic in nature. We performed RNA-Seq on developing flower and fruit samples of normal and mantled oil palm to characterize their transcriptomes. We present expression data for all transcripts in normal and mantled flower and fruit samples. Many genes are differentially expressed, including several from pathways that may be the cause of the mantled phenotype if disrupted, such as genes involved in primary hormone responses, DNA replication and repair, chromatin remodeling and a gene involved in RNA mediated DNA methylation. In addition, the gene expression data for developing flower and fruit will serve as a valuable resource for oil palm genetics and genomic studies.