Schwann cell-like remyelination following transplantation of human umbilical cord blood (hUCB)-derived mesenchymal stem cells in dogs with acute spinal cord injury
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- 作者：Jae-Hoon Lee ; Wook-Hun Chung ; Eun-Hee Kang ; Dai-Jung Chung ; Chi-Bong Choi ; Hwa-Seok Chang ; Jong-Hwan Lee ; Soo-Han Hwang ; Hoon Han ; Bo-Young Choe ; Hwi-Yool Kim
- 关键词：hUCB-MSC ; Remyelination ; P0 ; Transplantation ; Spinal cord injury ; Dog
- 刊名：Journal of the Neurological Sciences
- 出版年：2011
- 出版时间：15 January 2011
- 年：2011
- 卷：300
- 期：1-2
- 页码：86-96
- 全文大小：4165 K
文摘
Human umbilical cord blood derived mesenchymal stem cells (hUCB-MSCs) have significant therapeutic potential in cell-based therapies following spinal cord injury (SCI). To evaluate this potential, we conducted our preliminary investigations on the remyelination of injured spinal cords with hUCB-MSC transplantations and we observed its long term effects on dogs with SCI. Of the ten injured dogs, seven were transplanted with hUCB-MSCs 1 week after SCI, whereas the remaining three dogs were not transplanted. Two transplanted dogs died over the first month after transplantation because of urinary tract infection, bedsores and sepsis. The SCI dogs showed no improvement in motor and sensory functions and their urinary dysfunction persisted until they were euthanized (from 3 months to 1 year) while hind-limb recovery in 4 dogs among the five transplanted dogs was significantly improved. In the recovered dogs, functional recovery was sustained for three years following transplantation. Histological results from five transplanted dogs showed that many axons were remyelinated by P0-postive myelin sheaths after transplantation. Our results suggest that transplantation of hUCB-derived MSCs may have beneficial therapeutic effects. Furthermore, histological results provided the first in vivo evidence that hUCB-MSCs are able to enhance the remyelination of peripheral-type myelin sheaths following SCI.