Quantitative assessment of neurochemical changes in a rat model of long-term alcohol consumption as detected by in vivo and ex vivo proton nuclear magnetic resonance spectroscopy

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• 作者：Do-Wan Lee^a ; ^b ; Sang-Young Kim^a ; ^b ; Jae-Hwa Kim^c ; Taehyeong Lee^d ; Changbum Yoo^d ; Yoon-Ki Nam^e ; Jin-Young Jung^a ; ^b ; Hae-Chung Shin^e ; Hwi-Yool Kim^d ; Dai-Jin Kim^c ; ^f ; Bo-Young Choe^a ; ^b ; ^{bychoe@catholic.ac.kr}
• 关键词：MRS ; magnetic resonance spectroscopy ; NMRS ; nuclear magnetic resonance spectroscopy ; HR-MAS ; high-resolution magic angle spinning ; ppm ; part per million ; CPMG ; Carr-Purcell-Meiboom-Gill ; CRLB ; Cramé ; r&ndash ; Rao lower bound ; LCModel ; Linear Combinati
• 刊名：Neurochemistry International
• 出版年：2013
• 出版时间：March, 2013
• 年：2013
• 卷：62
• 期：4
• 页码：502-509
• 全文大小：672 K

文摘

The aim of present study was to quantitatively investigate the neurochemical profile of the frontal cortex region in a rat model of long-term alcohol consumption, by using in vivo proton magnetic resonance spectroscopy (¹H-MRS) at 4.7 T and ex vivo ¹H high-resolution magic angle spinning (HR-MAS) technique at 11.7 T. Twenty male rats were divided into two groups and fed a liquid diet for 10 weeks. After 10 weeks, in vivo ¹H MRS spectra were acquired from the frontal cortex brain region. After in vivo ¹H MRS experiments, all animals were sacrificed and 20 frontal cortex tissue samples were harvested. All tissue examinations were performed with the 11.7 T HR-MAS spectrometer and high-resolution spectra were acquired. The in vivo and ex vivo spectra were quantified as absolute metabolite concentrations and normalized ratios of total signal-intensity (i.e., metabolites_Norm), respectively. The absolute quantifications of in vivo spectra showed significantly higher glycerophosphocholine plus phosphocholine (GPC + PCh) and lower myo-inositol (mIns) concentrations in ethanol-treated rats compared to controls. The quantifications of ex vivo spectra showed significantly higher PCh_Norm, Cho_Norm and tCho_Norm, and lower GPC_Norm and mIns_Norm ratio levels in ethanol-treated rats compared to controls. Our findings suggest that reduced mIns concentrations caused by the long-term alcohol consumption may lead to hypo-osmolarity syndrome and astrocyte hyponatremia. In addition, increased choline-containing compound concentrations may reflect an increased cell turnover rate of phosphatidylcholine and other phospholipids, indicating an adaptive mechanism. Therefore, these results might be utilized as key markers in chronic alcohol intoxication metabolism.