Screening test for rapid food safety evaluation by menadione-catalysed chemiluminescent assay

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• 作者：Shiro Yamashoji ; ^{yamasho@ms.sist.ac.jp} ; Naoko Yoshikawa ; Masayuki Kirihara ; Toshihiro Tsuneyoshi
• 关键词：Menadione ; Chemiluminescent assay ; Viable cell number ; Food safety evaluation
• 刊名：Food Chemistry
• 出版年：2013
• 出版时间：15 June, 2013
• 年：2013
• 卷：138
• 期：4
• 页码：2146-2151
• 全文大小：437 K

文摘

The chemiluminescent assay of menadione-catalysed H₂O₂ production by living mammalian cells was proposed to be useful for rapid food safety evaluation. The tested foods were extracted with water, ethanol and dimethylsulfoxide, and each extract was incubated with NIH3T3, Neuro-2a and HepG2 cells for 4 h. Menadione-catalysed H₂O₂ production by living mammalian cells exposed to each extract was determined by the chemiluminescent assay requiring only 10 min, and the viability of the cells was estimated as percentage based on H₂O₂ production by intact cells. In this study the cytotoxicity of food was rated in order of inhibitory effect on H₂O₂ production by intact cells. The well known natural toxins such as Fusarium mycotoxin, tomato toxin tomatine, potato toxin solanine and marine toxins terodotoxin and brevetoxin could be detected by the above chemiluminescent assay.