Knockdown of ZEB1, a master epithelial-to-mesenchymal transition (EMT) gene, suppresses anchorage-independent cell growth of lung cancer cells
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- 作者：Yoshihiro Takeyama ; Mitsuo Sato ; Mihoko Horio ; Tetsunari Hase ; Kenya Yoshida ; Toshihiko Yokoyama ; Harunori Nakashima ; Naozumi Hashimoto ; Yoshitaka Sekido ; Adi F. Gazdar ; John D. Minna ; Masashi Kondo
- 关键词：Lung cancer ; Epidermal growth factor receptor ; Anchorage-independent growth ; EMT ; MicroRNA ; RNA interference
- 刊名：Cancer Letters
- 出版年：2010
- 出版时间：28 October 2010
- 年：2010
- 卷：296
- 期：2
- 页码：216-224
- 全文大小：847 K
文摘
We found that among four master epithelial-to-mesenchymal transition (EMT)-inducing genes (ZEB1, SIP1, Snail, and Slug) ZEB1expression was most significantly correlated with the mesenchymal phenotype (high Vimentin and low E-cadherin expression) in non-small cell lung cancer (NSCLC) cell lines and tumors. Furthermore, ZEB1 knockdown with RNA interference in three NSCLC cell lines with high ZEB1 expression suppressed to varying degrees mass culture growth and liquid colony formation but in all cases dramatically suppressed soft agar colony formation. In addition, ZEB1 knockdown induced apoptosis in one of the three lines, indicating that the growth inhibitory effects of ZEB1 knockdown occurs in part through the activation of the apoptosis pathway. These results suggest that inhibiting ZEB1 function may be an attractive target for NSCLC therapeutic development.