The role of Pvs28 in sporozoite development in Anopheles sinensis and its longevity in BALB/c mice

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• 作者：Tong-Soo Kim^a ; ^c ; ¹ ; Hyung-Hwan Kim^b ; ^e ; ¹ ; Sung-Ung Moon^c ; ¹ ; Sun-Sim Lee^c ; E-Hyun Shin^d ; Chang-Mi Oh^c ; Yoon-Joong Kang^e ; ^f ; Do-Kyung Kim^e ; ^f ; Youngjoo Sohn^g ; Hyuck Kim^f ; Hyeong-Woo Lee^c ; ^h ; ^{rainlee67@yahoo.co.kr}
• 关键词：Plasmodium vivax ; Ookinete surface protein ; Transmission-blocking vaccine ; Monoclonal antibody ; Longevity
• 刊名：Experimental Parasitology
• 出版年：2011
• 出版时间：February 2011
• 年：2011
• 卷：127
• 期：2
• 页码：346-350
• 全文大小：272 K

文摘

To develop a vivax malaria vaccine for blocking malarial transmission, the ookinete surface protein Pvs28 was cloned from Korean malaria patients using polymerase chain reaction. The Pvs28 gene consists of 726 bp and encodes 241 amino acids. It was subcloned into the expression vector pQE30 and expressed in Escherichia coli. The expressed recombinant protein, rPvs28, has a molecular weight of about 28 kDa in SDS–PAGE analysis. A monoclonal antibody against rPvs28 was produced using BALB/c mice. It inhibited sporozoite development in Anopheles sinensis mosquitoes (n = 81) which is one of the malaria vectors in Korea, with relatively high antibody titer against rPv28 persisting for more than 6 months. These results indicate that rPvs28 induces an immune response in mice that effectively blocks sporozoite development in mosquitoes. Therefore it could be a vaccine candidate for preventing vivax malaria in Korea.