Design, synthesis and biological evaluation of novel 4-anilinoquinazolines with C-6 urea-linked side chains as inhibitors of the epidermal growth factor receptor

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• 作者：Xu Zhang ; Ting Peng ; Xun Ji ; Jian Li ; Linjiang Tong ; Zeng Li ; Wei Yang ; Yungen Xu ; Mengyuan Li ; Jian Ding ; Hualiang Jiang ; Hua Xie ; Hong Liu
• 关键词：EGFR inhibitor ; EGFR T790M/L858R ; Binding mode ; Irreversible EGFR inhibitor
• 刊名：Bioorganic and Medicinal Chemistry
• 出版年：15 December, 2013
• 年：2013
• 卷：21
• 期：24
• 页码：7988-7998
• 全文大小：3239 K

文摘

A novel series of anilinoquinazoline compounds with C-6 urea-linked side chains was designed and synthesized as reversible inhibitors of epidermal growth factor receptor (EGFR) based on the structure-activity relationships (SARs) of anilinoquinazoline inhibitors. All compounds demonstrated good inhibition of EGFR wild type (EGFR wt) (IC₅₀ = 0.024-1.715 渭M) and inhibited proliferation of A431cell line (IC₅₀ = 0.116-22.008 渭M). The binding mode of compounds 8a, 8d, 8k and 8o was consistent with the biological results. Moreover, compounds 8k and 8l almost completely blocked the phosphorylation of EGFR in A431 cell line at 0.01 渭M. Interestingly, all of the compounds also demonstrated moderate inhibition of EGFR/T790M/L858R (IC₅₀ = 0.049-5.578 渭M). In addition, compounds 8f and 8h blocked the autophosphorylation of EGFR in NCI-H1975 cells at high concentration (10 渭M), and compound 8f was confirmed to be an irreversible inhibitor through the dilution method. Importantly, the compounds with C-6 urea-linked side chains which did not contain Michael acceptors demonstrated moderate to strong irreversible EGFR inhibition.