Formulation and evaluation of targeted nanoparticles for breast cancer theranostic system

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• 作者：Pegah Dadras^a ; Fatemeh Atyabi^a ; ^b ; Shiva Irani^c ; Leila Ma'mani^d ; Alireza Foroumadi^e ; Zahra Hadavand Mirzaie^f ; Marzieh Ebrahimi^g ; R. Dinarvand^a ; ^b ; ^{dinarvand@tums.ac.ir}
• 关键词：Theranostics ; SPION ; Metastatic breast cancer ; Stem cell ; Docetaxel ; Silibinin
• 刊名：European Journal of Pharmaceutical Sciences
• 出版年：2017
• 出版时间：15 January 2017
• 年：2017
• 卷：97
• 期：Complete
• 页码：47-54
• 全文大小：1318 K
• 卷排序：97

文摘

Theranostic polymeric NPs developed for both cancer diagnosis and cancer therapy. This multifunctional polymeric vehicle was prepared by a single emulsion evaporation method, using carboxyl-terminated PLGA. LHRH as a targeting moiety, was conjugated to the surface of polymeric carrier by applying polyethylene glycol. The results indicated that the diameter of NPs was ~ 185.4 ± 4.6 nm as defined by DLS. The entrapment efficacy of docetaxel, silibinin, and SPIONs was 84.6 ± 4.1%, 80.6 ± 2.7%, and 77.9 ± 4.3%, respectively. The NPs showed a triphasic in-vitro drug release pattern. MTT assay was done on two cell lines, MCF-7 and SKOV-3. Enhanced cellular uptake ability of the targeted NPs to MCF-7 was evaluated in-vitro by confocal laser scanning microscopy. The results indicated that compared to non-targeted NPs, the LHRH targeted NPs had significant efficacy at IC50 concentration. The effect of the NPs on VEGF expression in MCF-7 and SKOV-3 cells was investigated by Real-Time PCR method. VEGF mRNA level expression in MCF-7 cell line reduced by 83% in comparison to control cell line. The designed NPs can be used as promising multifunctional platform for detection and targeted drug delivery in breast cancer.