Antifungal Activity of Aspidin BB from Dryopteris fragrans against Trichophyton rubrum Involved Inhibition of Ergosterol Biosynthesis

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• 作者：Shi-hao Yang^a ; Wen-hao Chen^a ; Feng Shan^b ; Xiao-zhou Jia^a ; Rong-rong Deng^a ; Chu-ping Tang^a ; Zhi-bin Shen^a ; ^{szb8113@126.com}
• 关键词：Aspidin BB ; Dryopteris fragrans ; ergosterol ; squalene epoxidase ; Trichophyton rubrum
• 刊名：Chinese Herbal Medicines
• 出版年：2017
• 出版时间：January 2017
• 年：2017
• 卷：9
• 期：1
• 页码：63-68
• 全文大小：536 K
• 卷排序：9

文摘

Aspidin BB, a typical phloroglucinol derivative from Dryopteris fragrans, possesses significant antifungal property. This study aimed to investigate potential mechanism of antifungal activity of Aspidin BB against Trichophyton rubrum which is the most common pathogens responsible for chronic dermatophytosis.MethodsThe minimum inhibitory concentration (MIC) of Aspidin BB against strains was determined by broth microdilution. The effects of Aspidin BB on ergosterol biosynthesis were investigated by content determination based on UPLC method. Besides, the effects of drugs on squalene epoxidase (SE) in T. rubrum cell membrane were analyzed.ResultsMIC value of Aspidin BB against T. rubrum was 25.0 μg/mL. Aspidin BB reduced ergosterol content significantly, but no notable effect on squalene epoxidase activity.ConclusionThe results suggested that Aspidin BB inhibited ergosterol biosynthesis. However, it was not squalene epoxidase but other components may sever as possible targets in ergosterol biosynthesis pathway.