- 作者：Biljana Ljujic ; Gordana Radosavljevic ; Ivan Jovanovic ; Sladjana Pavlovic ; Nemanja Zdravkovic ; Marija Milovanovic ; Ljubisa Acimovic ; Milan Knezevic ; Dragic Bankovic ; Dusica Zdravkovic ; Nebojsa Arsenije
- 关键词：IL-23 ; VEGF ; p53 ; Colorectal carcinoma
- 刊名：Archives of Medical Research
- 出版年：2010
- 出版时间：April 2010
- 年：2010
- 卷：41
- 期：3
- 页码：182-189
- 全文大小：444 K
Background and Aims
Interleukin-23 (IL-23) has a role in inflammatory bowel diseases (IBD) as a condition of higher risk of colorectal carcinogenesis. Vascular endothelial growth factor (VEGF) is overexpressed in IBD and colorectal carcinoma. Therefore, we aimed at uncovering the relationship between serum level of IL-23 and expression of VEGF in colorectal cancer (CRC) patients and to establish the relationship between VEGF and p53 and serum levels of IL-23, as well as its possible role in carcinogenesis of colorectal carcinomas.Methods
Levels of IL-23 from serum samples of patients with colorectal carcinoma (n = 40) and healthy control samples (n = 37) were examined for IL-23-Ab using an ELISA assay. We also determined the expression of VEGF and p53 by immunohistochemistry in 59 cases of CRC.
Results
We found significantly higher serum levels of IL-23 in patients with CRC compared to control subjects (IL-23; mean 189.46 pg/mL vs. mean 34.77 pg/mL, p = 0.033). We also detected higher serum levels of IL-23 in patients with overexpressed VEGF (p = 0.028). Our results also showed that concomitant expression of VEGF and increased serum levels of IL-23 are in positive correlation with histological grade 2 (p <0.05).
Conclusions
Our data indicate that serum IL-23 levels are significantly elevated in CRC vs. control patients and are strongly associated with overexpression of VEGF, thus they may play an important role in carcinogenesis of CRC.