Rapid structural characterization of triterpenoid saponins in crude extract from Symplocos chinensis using liquid chromatography combined with electrospray ionization tandem mass spectrometry

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• 作者：Bin Li ; Zeper Abliz ; Meijun Tang ; Guangmiao Fu and Shishan Yu
• 关键词：Symplocos chinensis ; Triterpenoid saponins ; Analytical method ; Positive and negative ion ESI– ; MSⁿ ; LC– ; MSⁿ
• 刊名：Journal of Chromatography A
• 出版年：2006
• 出版时间：6 January 2006
• 年：2006
• 卷：1101
• 期：1-2
• 页码：53-62
• 全文大小：386 K

文摘

Triterpenoid saponins in bioactive crude extract from Symplocos chinensis were rapidly identified using electrospray ionization multi-stage tandem mass spectrometry (ESI–MSⁿ) and liquid chromatography coupled with sequential mass spectrometry (LC–MSⁿ). According to the characteristic fragmentation behavior of known glucuronide-type triterpenoid saponins isolated from this plant, a total of fourteen constituents in the crude extract were structurally characterized on the basis of their retention time and tandem mass spectrometric analysis, including five pairs of naturally occurring isomers. Except one known saponin formerly obtained, the other constituents were new compounds. The analytical method of LC–MSⁿ combined with ESI–MSⁿ in positive and negative ion modes has been developed for the direct structural elucidation of triterpenoid saponins of this kind in plant extracts.