Kinetically controlled syntheses catalyzed by proteases in reverse micelles and separation of precursor dipeptides of RGD

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• 作者：Chen ; Yu-Xin ; Zhanga ; Xue-Zhong ; Chen ; Song-Ming ; You ; De-Lin ; Wu ; Xiao-Xia ; Yang ; Xue-Cheng ; et. al.
• 关键词：Dipeptide syntheses ; Proteases ; Reverse micelles ; Organic solvent
• 刊名：Enzyme and Microbial Technology
• 出版年：1999
• 出版时间：August, 1999
• 年：1999
• 卷：25
• 期：3-5
• 页码：310-315
• 全文大小：160 K

文摘

The precursor dipeptides of RGD (Arg-Gly-Asp), N-CBZ-Arg-Gly-NH₂ and CBZ-Gly-Asp-NH₂ were synthesized in reverse micellar system and organic solvent using several proteases in different forms under kinetical control. The synthesis reaction conditions for CBZ-Arg-Gly-NH₂ and CBZ-Gly-Asp-NH₂ catalyzed by chymotrypsin in AOT/isooctane reverse micelles were optimized by examining the effects of several factors including water content, temperature, pH, and reaction time on the dipeptide yields. The best yields for the syntheses of CBZ-Arg-Gly-NH₂ and CBZ-Gly-Asp-NH₂ were 87.3 % and 80.8 % , respectively. The dipeptide products were separated from the reverse micelles by using a silica gel column.