Cell-free miR-24 and miR-30d, potential diagnostic biomarkers in malignant effusions

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• 作者：Li Xie^a ; ^b ; Tingting Wang^a ; ^b ; Shaorong Yu^a ; ^b ; Xi Chen^c ; Lifeng Wang^a ; ^b ; Xiaoping Qian^a ; ^b ; Lixia Yu^a ; ^b ; Yitao Ding^d ; Chenyu Zhang^c ; ^{zhangchenyu_nju@163.com} ; Baorui Liu^a ; ^b ; ^d ; ^{baoruiliu@nju.edu.cn}
• 关键词：Malignant effusions ; Cell-free miRNAs ; miR-24 ; miR-30d ; Diagnosis
• 刊名：Clinical Biochemistry
• 出版年：2011
• 出版时间：February 2011
• 年：2011
• 卷：44
• 期：2-3
• 页码：216-220
• 全文大小：364 K

文摘

Objectives

The study was trying to figure out the possible role of cell-free miR-24 and miR-30d as diagnostic biomarkers for malignant effusions.

Methods

A total of 110 effusion samples (28 benign and 82 malignant) were collected and cell-free total RNA was extracted. Quantitative RT-PCR was carried out to measure the expression levels of miR-24 and miR-30d, with an exogenous supplemented plant miRNA, ath-miR156a as the reference RNA.

Results

Malignant pleural effusions had higher expression levels of cell-free miR-24 and miR-30d than benign effusions. In the ascites group, only miR-30d was found to be significantly up-regulated in the supernatant of effusions. Combination of cell-free miR-24 and miR-30d could discriminate malignant ascites from benign with improved potency comparing to single miR-30d.

Conclusions

Cell-free miR-30d and miR-24 are potential diagnostic biomarkers for malignant and benign effusions.