Membrane cartridges for endotoxin removal from interferon preparations

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• 作者：Li ; Jinghua ; Shao ; Yingguang ; Chen ; Zhaoan ; Cong ; Runzi ; Wang ; Junde ; Liu ; Xueliang
• 关键词：Membrane cartridges ; Cellulose membrane ; Interferon ; Chitosan ; Deoxycholate ; Endotoxin
• 刊名：Journal of Chromatography B, Analytical Technologies in the Biomedical and Life Sciences
• 出版年：2003
• 出版时间：July 5, 2003
• 年：2003
• 卷：791
• 期：1-2
• 页码：55-61
• 全文大小：149 K

文摘

The suitability of membrane cartridges for the removal of endotoxin from both distilled water and interferon preparations was examined. The endotoxin concentrations were reduced to 4.0 and 7.3 EU/ml, respectively, when about 4000 ml of distilled water with 20 and 28 EU/ml were passed through the deoxycholate and chitosan immobilized membrane cartridges. When 200 ml of interferon preparation with endotoxin concentration more than 80 EU/ml and pH 3.9 were applied to a deoxycholate immobilized membrane cartridge at a flow-rate of 9 ml/min, the endotoxin concentration was reduced to less than 10 EU/ml. However, if an interferon preparation of 450 ml, with more than 80 EU/ml of endotoxin and pH 3.9 was applied to the chitosan immobilized membrane cartridge at a flow-rate of 18 ml/min, the endotoxin concentration was reduced to less than 10 EU/ml.