The rescue and evaluation of FLAG and HIS epitope-tagged Asia 1 type foot-and-mouth disease viruses
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- 作者：Bo Yang ; Fan Yang ; Yan Zhang ; Huanan Liu ; Ye Jin ; Weijun Cao ; Zixiang Zhu ; Haixue Zheng ; haixuezheng@163.com" class="auth_mail" title="E-mail the corresponding author ; Hong Yin
- 关键词：Foot-and-mouth disease virus (FMDV) ; Reverse genetic ; Recombinant ; Marker vaccine
- 刊名：Virus Research
- 出版年：2016
- 出版时间：2 February 2016
- 年：2016
- 卷：213
- 期：Complete
- 页码：246-254
- 全文大小：2370 K
文摘
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Two epitope-tagged viruses infectious clone with foreign epitope in the RGD + 9 of VP1 G–H loop were generated indicated RGD + 9 also can be regarded as an insertion for foreign genes.
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The foreign epitope can be stably expressed on the rescued virus after passage in BHK-21 cells.
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The insertion have no impact on the antigenicity of the FMDV both in vitro and in vivo.
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These results laid the foundation for the distinguishing between vaccinated and FMDV-infected animals and the development of novel FMDV vaccine.