DHX9/RHA Binding to the PBS-Segment of the Genomic RNA during HIV-1 Assembly Bolsters Virion Infectivity
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- 作者：Ioana Boeras1 ; &dagger ; ; Zhenwei Song2 ; &dagger ; ; Andrew Moran2 ; Jarryd Franklin2 ; William Clay Brown3 ; Marc Johnson4 ; Kathleen Boris-Lawrie1 ; kbl@umn.edu" class="auth_mail" title="E-mail the corresponding author ; Xiao Heng2 ; hengx@missouri.edu" class="auth_mail" title="E-mail the corresponding author
- 关键词：HIV-1 ; human immunodeficiency virus type 1 ; RBP ; RNA-binding protein ; RHA ; RNA helicase A/DHX9 ; gRNA ; genomic RNA ; dsRBDs ; dsRNA-binding domainsx ; 5&prime ; -UTR ; 5&prime ; -untranslated region ; gRNP ; genomic ribonucleoprotein particles ; NC ; nucleocapsid ; CES ; core encapsidation signal ; PBS ; primer binding site ; RRE ; Rev response element ; TAR ; transactivation responsive sequence ; ITC ; isothermal titration calorimetry ; NOESY ; NOE spectroscopy ; PAS ; primer activation signal
- 刊名：Journal of Molecular Biology
- 出版年：2016
- 出版时间：5 June 2016
- 年：2016
- 卷：428
- 期：11
- 页码：2418-2429
- 全文大小：1168 K
文摘
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Host DHX9/RHA is recruited in HIV-1 virions to support infectivity.
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Cell-based assays identified the 5′-UTR necessary to RHA recruitment to virions.
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NMR data of the complex (290 kDa) pinpointed PBS as the RHA-binding site.
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A PBS point mutation that precluded RHA binding attenuated virion infectivity.
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Our work sheds light on the RHA involvement in the early viral replication events.