Reactive oxygen species acts as executor in radiation enhancement and autophagy inducing by AgNPs

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• 作者：Hao Wu^a ; Jun Lin^b ; Peidang Liu^a ; ^c ; Zhihai Huang^a ; Peng Zhao^a ; Haizhen Jin^c ; Jun Ma^d ; Longping Wen^b ; ¹ ; ^{lpwen@ustc.edu.cn" class="auth_mail" title="E-mail the corresponding author} ; Ning Gu^a ; ¹ ; ^{guning@seu.edu.cn" class="auth_mail" title="E-mail the corresponding author}
• 关键词：Silver nanoparticles ; Autophagy ; Glioma ; Radiotherapy ; Radiation sensitization ; Reactive oxygen species (ROS)
• 刊名：Biomaterials
• 出版年：2016
• 出版时间：September 2016
• 年：2016
• 卷：101
• 期：Complete
• 页码：1-9
• 全文大小：2658 K

文摘

Malignant glioma is one of the most common intracranial tumor with a dismal prognosis. The radiosensitizing effect of silver nanoparticles (AgNPs) on glioma both in vitro and in vivo were demonstrated in the previous studies of our group. However, the underlying mechanism is still unclear. In this present study, the use of antioxidants is employed for the regulating of reactive oxygen species (ROS) in U251 cells treated with various agents, and the results shows that ROS played an essential role in the autophagy inducing and radiosensitization effect of AgNPs. Moreover, the inhibition of protective autophagy with 3-MA is another way to increase ROS, resulting in the increasing of cell death and apoptosis. Taken together, understanding the relationship between the elevated ROS and autophagy and the effect of ROS should be useful to the clinical applications of AgNPs. These findings could potentially be exploited for new therapeutic strategies in glioma radiotherapy.