Fe3O4@Ag magnetic nanoparticles for microRNA capture and duplex-specific nuclease signal amplification based SERS detection in cancer cells
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- 作者：Yuanfeng Panga ; b ; Chongwen Wangb ; c ; Jing Wangb ; d ; Zhiwei Suna ; zwsun@ccmu.edu.cn" class="auth_mail" title="E-mail the corresponding author ; Rui Xiaob ; Ruixiao203@sina.com" class="auth_mail" title="E-mail the corresponding author ; Shengqi Wangb ;
- 关键词：MiRNA ; Duplex-specific nuclease ; Fe3O4@Ag ; Paramagnetic ; SERS
- 刊名：Biosensors and Bioelectronics
- 出版年：2016
- 出版时间：15 May 2016
- 年：2016
- 卷：79
- 期：Complete
- 页码：574-580
- 全文大小：1220 K
文摘
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The signal amplification and single-base discrimination ability of DSN were well combined with the high SERS performance of the Fe3O4@Ag.
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MiRNA let-7b was captured, concentrated and quantified from RNAt of cancer cells within a PE tube with a detection limit of 0.3 fM, nearly 3 orders of magnitude lower than fluorescence based DSN biosensor for miRNA.
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The result provides a proposal to apply metal complex magnetic microsphere as novel platform to combine miRNAs capture, concentration and single-base level SERS detection in one step.