A new NOE-mediated MT signal at around − 1.6 ppm for detecting ischemic stroke in rat brain

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• 作者：Xiao-Yong Zhang^a ; ^b ; Feng Wang^a ; ^b ; Aqeela Afzal^c ; Junzhong Xu^a ; ^b ; ^d ; ^e ; John C. Gore^a ; ^b ; ^d ; ^e ; ^f ; Daniel F. Gochberg^a ; ^b ; ^d ; Zhongliang Zu^a ; ^b ; ^{zhongliang.zu@vanderbilt.edu" class="auth_mail" title="E-mail the corresponding author}
• 关键词：Magnetization transfer (MT) ; Chemical exchange saturation transfer (CEST) ; Nuclear Overhauser enhancement (NOE) ; Ischemic stroke
• 刊名：Magnetic Resonance Imaging
• 出版年：2016
• 出版时间：October 2016
• 年：2016
• 卷：34
• 期：8
• 页码：1100-1106
• 全文大小：1244 K

文摘

In the present work, we reported a new nuclear Overhauser enhancement (NOE)-mediated magnetization transfer (MT) signal at around − 1.6 ppm (NOE(− 1.6)) in rat brain and investigated its application in the detection of acute ischemic stroke in rodent model. Using continuous wave (CW) MT sequence, the NOE(− 1.6) is reliably detected in rat brain. The amplitude of this new NOE signal in rat brain was quantified using a 5-pool Lorentzian Z-spectral fitting method. Amplitudes of amide, amine, NOE at − 3.5 ppm (NOE(− 3.5)), as well as NOE(− 1.6) were mapped using this fitting method in rat brain. Several other conventional imaging parameters (R₁, R₂, apparent diffusion coefficient (ADC), and semi-solid pool size ratio (PSR)) were also measured. Our results show that NOE(− 1.6), R₁, R₂, ADC, and APT signals from stroke lesion have significant changes at 0.5–1 h after stroke. Compared with several other imaging parameters, NOE(− 1.6) shows the strongest contrast differences between stroke and contralateral normal tissues and stays consistent over time until 2 h after onset of stroke. Our results demonstrate that this new NOE(− 1.6) signal in rat brain is a new potential contrast for assessment of acute stroke in vivo and might provide broad applications in the detection of other abnormal tissues.