Cultivation of Chlorella zofingiensis in bench-scale outdoor ponds by regulation of pH using dairy wastewater in winter, South China

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• 作者：Shuhao Huo ; Zhongming Wang ; Shunni Zhu ; Weizheng Zhou ; Renjie Dong ; Zhenhong Yuan
• 关键词：Microalgae ; pH adjustment ; Wastewater ; Biofuel ; ¦Ã-Aminobutyric acid
• 刊名：Bioresource Technology
• 出版年：2012
• 出版时间：October, 2012
• 年：2012
• 卷：121
• 期：Complete
• 页码：76-82
• 全文大小：529 K

文摘

Cultivation of Chlorella zofingiensis and nutrients removal in dairy wastewater were investigated in bench-scale outdoor ponds in winter, South China. The impacts of the two types of pH regulations, 5¡«6 % CO₂ and acetic acid (HAc) on this process were studied. After 6 days cultivation, the removal rates of total nitrogen (TN) and orthophosphate () using CO₂ regulation were better than those using HAc. The removal rates of and TN were 97.5 % and 51.7 % , respectively using CO₂ regulation; 79.6 % (TN) and 42.0 % () were obtained using HAc regulation. Higher biomass, protein, sugar content, and stable pH control were found using CO₂ regulation. However, significantly higher lipid content (31.8 % ) was observed using HAc regulation. The dominant differences of fatty acids were the content of C18:1 and C18:3. The growth characteristics and environmental conditions especially during the typical logarithmic phase were also analyzed.