Rapid and unbiased extraction of chromatin associated RNAs from purified native chromatin
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- 作者：Zhongwu Zhoua ; b ; c ; 1 ; Yi Yanga ; c ; d ; 1 ; Stephen F. Koniecznya ; c ; d ; Joseph M.K. Irudayaraja ; b ; c ; josephi@purdue.edu" class="auth_mail" title="E-mail the corresponding author
- 关键词：Unbiased preparation of chromatin associated RNAs ; Magnetic nanoparticle ; Magnetophoretic chromatography ; Heterochromatin ; Soluble chromatin
- 刊名：Journal of Chromatography A
- 出版年：2015
- 出版时间：24 December 2015
- 年：2015
- 卷：1426
- 期：Complete
- 页码：64-68
- 全文大小：1075 K
文摘
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Salicylic acid coated magnetic nanoparticles have dual functions wherein they can be used to enrich mammalian cells and further capture chromatin after cell lysis.
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Magnetophoretic chromatography in a viscous PEG solution removes free RNA from the chromatin.
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Compared to RNAs extracted from nucleus, non-coding RNAs can be significantly enriched with the magnetophoretic chromatography method.
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An ultra fast and unbiased method for chromatin associated RNA extraction including cell separation, chromatin purification and chromatin associated RNA extraction can be accomplished in less than 2 h.