Functional and Structural Insights of the Zinc-Finger HIT protein family members Involved in Box C/D snoRNP Biogenesis
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- 作者：Benoit Bragantini1 ; &dagger ; ; Decebal Tiotiu1 ; &dagger ; ; Benjamin Rothé ; 1 ; 5 ; Jean-Michel Saliou3 ; 4 ; 6 ; Hé ; lè ; ne Marty1 ; Sarah Cianfé ; rani3 ; 4 ; Bruno Charpentier1 ; Marc Quinternet2 ; marc.quinternet@univ-lorraine.fr" class="auth_mail" title="E-mail the corresponding author ; Xavier Manival1 ; xavier.manival@univ-lorraine.fr" class="auth_mail" title="E-mail the corresponding author
- 关键词：ZnF ; zinc-finger ; ZHD ; zf&ndash ; HIT domain ; AP-MS ; affinity purification and mass spectrometry ; TRIP3 ; thyroid hormone receptor interacting protein 3 ; snoRNP ; small nucleolar ribonucleoprotein ; ZnF1 ; first zinc finger ; ZnF2 ; second zinc finger ; HSQC ; heteronuclear single quantum coherence ; NOESY ; nuclear Overhauser enhancement spectroscopy ; Bcd1p-FL ; full-length Bcd1p ; WT ; wild-type ; EMSA ; electrophoresis mobility shift assays ; RSV ; Rous sarcoma virus ; SelN ; Selenoprotein N
- 刊名：Journal of Molecular Biology
- 出版年：2016
- 出版时间：5 June 2016
- 年：2016
- 卷：428
- 期：11
- 页码：2488-2506
- 全文大小：1738 K
文摘
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ZHD of Bcd1p contributes to the steady-state level of the box C/D snoRNAs and is essential for cellular viability.
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The 3D solution structure of ZHDs of Bcd1p and Hit1p was determined by NMR.
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Bcd1p ZDH is functionally interchangeable with Hit1p ZDH, another box C/D snoRNP assembly factor.
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Mutation of the ZnF2 motif in the ZHD of Bcd1p induces a heat-sensitive phenotype in yeast.
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The Bcd1p ZHD is a functional fold stabilizer.