Mutational analysis of human prothymosin α reveals a bipartite nuclear localization signal
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- 作者：Rubtsov ; Yuri P. ; Zolotukhin ; Andrei S. ; Vorobjev ; Ivan A. ; Chichkova ; Nina V. ; Pavlov ; Nickolay A. ; et. al.
- 关键词：Prothymosin α ; PCR-based mutagenesis ; Cell growth inhibition ; Nuclear localization signal ; Saccharomyces cerevisiae ; ProTα ; prothymosin α ; bp ; base pair(s) ; CMV ; cytomegalovirus ; GFP ; green fluorescent protein ; NLS ; nuclear localization sig
- 刊名：FEBS Letters
- 出版年：1997
- 出版时间：August 11, 1997
- 年：1997
- 卷：413
- 期：1
- 页码：135-141
- 全文大小：779 K
文摘
Mutants of human prothymosin α with impaired ability to inhibit yeast Saccharomyces cerevisiae. cerevisiae cell growth were characterized. Two types of prothymosin α-inactivating mutations were observed. Mutations that belong to the first type compromised the nuclear entry of prothymosin α by affecting its nuclear localization signal. Analysis of subcellular distribution of GFP-prothymosin α fusions revealed a bipartite nuclear localization signal that is both necessary and sufficient for nuclear import of the protein in human cells. Mutations of the second type abrogated the inhibitory action of prothymosin α through an unknown mechanism, without influencing the nuclear import of the protein.