IL-8 Release from Human Neutrophils Cultured with Pro-Haptenic Chemical Sensitizers

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• 作者：Eva Kiorpelidou ; Brian Foster ; John Farrell ; Monday O. Ogese ; Lee Faulkner ; Chris E. Goldring ; B. Kevin Park ; Dean J. Naisbitt
• 刊名：Chemical Research in Toxicology
• 出版年：2012
• 出版时间：October 15, 2012
• 年：2012
• 卷：25
• 期：10
• 页码：2054-2056
• 全文大小：181K
• 年卷期：v.25,no.10(October 15, 2012)
• ISSN：1520-5010

文摘

Cytokine release from dendritic cells in vitro is a useful marker to discriminate between sensitizing and irritant haptenic chemicals. Unfortunately, pro-haptens, which gain reactivity following metabolic/auto activation, yield negative results. To overcome this, we exposed human neutrophils and THP-1 cells to haptens/pro-haptens and measured IL-8 release. Haptenic compounds stimulated IL-8 release in neutrophils and THP-1 cells. In contrast, the pro-haptens eugenol, isoeugenol, and 2-aminophenol stimulated high levels of IL-8 release from neutrophils alone. Neutrophil cytokine release was reduced when glutathione was added. Cyp1A1/1B1/3A4 were not detectable in THP-1 cells or neutrophils; however, neutrophils expressed high levels of myeloperoxidase.