13C{13C} 2D NMR: A Novel Strategy for the Study of Paramagnetic Proteins with Slow Electronic Relaxation Rates

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• 作者：Timothy E. Machonkin ; William M. Westler ; and John L. Markley
• 刊名：Journal of the American Chemical Society
• 出版年：2002
• 出版时间：April 3, 2002
• 年：2002
• 卷：124
• 期：13
• 页码：3204 - 3205
• 全文大小：70K
• 年卷期：v.124,no.13(April 3, 2002)
• ISSN：1520-5126

文摘

Oxidized human [2Fe-2S] ferredoxin has a notably slow electronic relaxation rate, which precludes the observation of signals from nuclei near the iron-sulfur cluster by conventional 2D or 3D methods that utilize proton detection. We have demonstrated the utility of ¹³C{¹³C} CT-COSY in identifying connectivity information from fast relaxing carbon nuclei near the paramagnetic center, including those from residues that ligate the metal center.