文摘
Tomato is a good source of bioactive molecules such as vitamin C, carotenoids, and phenolic compounds. Up to now, only a few studies have evaluated the bioavailability of phenolic compounds from tomato. This paper presents the optimization of a method for the determination of phenolics in tomato and their metabolites in human urine and plasma after ingestion of tomato sauce. The sample preparation includes a SPE step to obtain cleaner extracts for injection in the LC-MS/MS system. The mean recovery of analytes ranged from 73 to 104% in plasma and from 65 to 106% in urine, the accuracy was between 90.3 and 115.0% in urine and between 85.7 and 115.0% in plasma, and the precision coefficient of variation was <15%. The method allowed detection and quantification limits of 0.5鈥?9 and 2.0鈥?0 ng mL鈥? in urine, respectively, and 0.5鈥?0 and 2.0鈥?05 ng mL鈥? in plasma, respectively, for the same phenolic compounds.
Keywords:
tomato; phenolic metabolites; LC-MS/MS; plasma; urine