Validation of a New LC-MS/MS Method for the Detection and Quantification of Phenolic Metabolites from Tomato Sauce in Biological Samples

详细信息    查看全文

• 作者：Miriam Martı ; &#769 ; nez-Hu茅lamo ; Sara Tulipani ; Xavier Torrado ; Ram贸n Estruch ; Rosa M. Lamuela-Ravent贸s
• 刊名：Journal of Agricultural and Food Chemistry
• 出版年：2012
• 出版时间：May 9, 2012
• 年：2012
• 卷：60
• 期：18
• 页码：4542-4549
• 全文大小：265K
• 年卷期：v.60,no.18(May 9, 2012)
• ISSN：1520-5118

文摘

Tomato is a good source of bioactive molecules such as vitamin C, carotenoids, and phenolic compounds. Up to now, only a few studies have evaluated the bioavailability of phenolic compounds from tomato. This paper presents the optimization of a method for the determination of phenolics in tomato and their metabolites in human urine and plasma after ingestion of tomato sauce. The sample preparation includes a SPE step to obtain cleaner extracts for injection in the LC-MS/MS system. The mean recovery of analytes ranged from 73 to 104% in plasma and from 65 to 106% in urine, the accuracy was between 90.3 and 115.0% in urine and between 85.7 and 115.0% in plasma, and the precision coefficient of variation was <15%. The method allowed detection and quantification limits of 0.5鈥?9 and 2.0鈥?0 ng mL^鈥? in urine, respectively, and 0.5鈥?0 and 2.0鈥?05 ng mL^鈥? in plasma, respectively, for the same phenolic compounds.

Keywords:

tomato; phenolic metabolites; LC-MS/MS; plasma; urine