In this work we have analyzed the structural, topographical, and shear characteristics of mixed monolayers formedby adsorbed
![](/images/gifchars/beta2.gif)
-lactoglobulin (
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-lg) and spread monoglyceride (monopalmitin or monoolein) on a previously adsorbedprotein film. Measurements of the surface pressure (
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)-area (
A) isotherm, Brewster angle microscopy (BAM), andsurface shear characteristics were obtained at 20
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C and at pH 7 in a modified Wilhelmy-type film balance. The
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-
Aisotherm and BAM images deduced for adsorbed
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-lactoglobulin-monoglyceride mixed films at
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lower than theequilibrium surface pressure of
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-lactoglobulin (
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) indicate that
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-lactoglobulin and monoglyceride coexist at theinterface. However, the interactions between protein and monoglyceride are somewhat weak. At higher surface pressures(at
![](/isubscribe/journals/langd5/23/i13/eqn/la7003497e10002.gif)
) a protein displacement by the monoglyceride from the interface takes place. The surface shear viscosity(
s) of mixed films is very sensitive to protein-monoglyceride interactions and displacement as a function of monolayercomposition (protein/monoglyceride fraction) and surface pressure. Shear can induce change in the morphology ofmonoglyceride and
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-lactoglobulin domains, on the one hand, and segregation between domains of the film-formingcomponents on the other hand. In addition, the displacement of
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-lactoglobulin by the monoglycerides is facilitatedunder shear conditions.