Searching Urinary Tumor Markers for Bladder Cancer Using a Two-Dimensional Differential Gel Electrophoresis (2D-DIGE) Approach

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• 作者：Esteban Orenes-Pi&ntilde ; ero ; Marta Cortó ; n ; Pilar Gonz&aacute ; lez-Peramato ; Ferr&aacute ; n Algaba ; Ignacio Casal ; Alvaro Serrano ; Marta S&aacute ; nchez-Carbayo
• 刊名：Journal of Proteome Research
• 出版年：2007
• 出版时间：November 2007
• 年：2007
• 卷：6
• 期：11
• 页码：4440 - 4448
• 全文大小：714K
• 年卷期：v.6,no.11(November 2007)
• ISSN：1535-3907

文摘

Aiming at identifying biomarkers for bladder cancer, the urinary proteome was explored through atwo-dimensional gel-based proteomic approach (2D-DIGE) coupled with mass spectrometry anddatabase interrogation. The increased expression of proteins differentially expressed between patientswith bladder tumors and controls such as Reg-1 and keratin 10 was confirmed to be associated withbladder cancer progression on bladder cancer cell lines by immunoblotting, and bladder tumors byimmunohistochemistry. Moreover, the association of these proteins, especially Reg-1, with tumor stagingand clinical outcome was confirmed by immunohistochemistry using an independent series of bladdertumors contained in tissue microarrays (n = 292). Furthermore, Reg-1 was quantified using anindependent series of urinary specimens (n = 80) and provided diagnostic utility to discriminate patientswith bladder cancer and controls (area under the curve (AUC = 0.88)). Thus, the 2D-DIGE approachhas identified Reg-1 as a biomarker for bladder cancer diagnostics, staging, and outcome prognosis.