Construction and Structure Determination of a Three-Dimensional DNA Crystal

详细信息    查看全文

• 作者：Chad R. Simmons ; Fei Zhang ; Jens J. Birktoft ; Xiaodong Qi ; Dongran Han ; Yan Liu ; Ruojie Sha ; Hatem O. Abdallah ; Carina Hernandez ; Yoel P. Ohayon ; Nadrian C. Seeman ; Hao Yan
• 刊名：Journal of the American Chemical Society
• 出版年：2016
• 出版时间：August 10, 2016
• 年：2016
• 卷：138
• 期：31
• 页码：10047-10054
• 全文大小：631K
• 年卷期：0
• ISSN：1520-5126

文摘

Structural DNA nanotechnology combines branched DNA junctions with sticky-ended cohesion to create self-assembling macromolecular architectures. One of the key goals of structural DNA nanotechnology is to construct three-dimensional (3D) crystalline lattices. Here we present a new DNA motif and a strategy that has led to the assembly of a 3D lattice. We have determined the X-ray crystal structures of two related constructs to 3.1 Å resolution using bromine-derivatized crystals. The motif we used employs a five-nucleotide repeating sequence that weaves through a series of two-turn DNA duplexes. The duplexes are tied into a layered structure that is organized and dictated by a concert of four-arm junctions; these in turn assemble into continuous arrays facilitated by sequence-specific sticky-ended cohesion. The 3D X-ray structure of these DNA crystals holds promise for the design of new structural motifs to create programmable 3D DNA lattices with atomic spatial resolution. The two arrays differ by the use of four or six repeats of the five-nucleotide units in the repeating but statistically disordered central strand. In addition, we report a 2D rhombuslike array formed from similar components.