Near-Infrared Spectrometry of Abdominal Aortic Aneurysm in the ApoE-/- Mouse

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• 作者：Aaron Urbas ; Michael W. Manning ; Alan Daugherty ; Lisa A. Cassis ; and Robert A. Lodder
• 刊名：Analytical Chemistry
• 出版年：2003
• 出版时间：July 15, 2003
• 年：2003
• 卷：75
• 期：14
• 页码：3650 - 3655
• 全文大小：100K
• 年卷期：v.75,no.14(July 15, 2003)
• ISSN：1520-6882

文摘

Abdominal aortic aneurysms (AAAs) occur in 5-7% ofpeople over age 60 in the United States. Early interventionin the disease process could have a significant impact onthe incidence of complications and on patient survival,but identifying incipient aneurysms can be difficult. ApoEknockout mice develop AAAs following infusion of angiotensin II (AngII) by osmotic minipump into the subcutaneous space of mice at doses ranging from 500 to 1000ng kg^-1 min^-1 for 7-28 days. These mice are used asmodels of AAA development. This study tested the hypothesis that near-IR spectrometry and PCR can determine AngII dose (SEE = 26 ng kg^-1 min^-1, SEP = 37 ngkg^-1 min^-1, r² = 0.99) and collagen/elastin (C/E) ratio(SEE = 0.38, SEP = 0.39, r² = 0.85) in mouse aortas.