Characterization of Folic Acid and Poly(amidoamine) Dendrimer Interactions with Folate Binding Protein: A Force-Pulling Study

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• 作者：Pascale R. Leroueil ; Stassi DiMaggio ; Abigail N. Leistra ; Craig D. Blanchette ; Christine Orme ; Kumar Sinniah ; Bradford G. Orr ; Mark M. Banaszak Holl
• 刊名：Journal of Physical Chemistry B
• 出版年：2015
• 出版时间：September 3, 2015
• 年：2015
• 卷：119
• 期：35
• 页码：11506-11512
• 全文大小：338K
• ISSN：1520-5207

文摘

Atomic force microscopy force-pulling experiments have been used to measure the binding forces between folic acid (FA) conjugated poly(amidoamine) (PAMAM) dendrimers and folate binding protein (FBP). The generation 5 (G5) PAMAM conjugates contained an average of 2.7, 4.7, and 7.2 FA per dendrimer. The most probable rupture force was measured to be 83, 201, and 189 pN for G5-FA_2.7, G5-FA_4.7, and G5-FA_7.2, respectively. Folic acid blocking experiments for G5-FA_7.2 reduced the frequency of successful binding events and increased the magnitude of the average rupture force to 274 pN. The force data are interpreted as arising from a network of van der Waals and electrostatic interactions that form between FBP and G5 PAMAM dendrimer, resulting in a binding strength far greater than that expected for an interaction between FA and FBP alone.