A Rational Approach to Minimal High-Resolution Cross-Reactive Arrays

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• 作者：Eric Green ; Mark J. Olah ; Tatiana Abramova ; Lance R. Williams ; Darko Stefanovic ; Tilla Worgall ; Milan N. Stojanovic
• 刊名：Journal of the American Chemical Society
• 出版年：2006
• 出版时间：November 29, 2006
• 年：2006
• 卷：128
• 期：47
• 页码：15278 - 15282
• 全文大小：151K
• 年卷期：v.128,no.47(November 29, 2006)
• ISSN：1520-5126

文摘

We report a rational approach to the construction of cross-reactive arrays for steroids consistingof five to seven sensors incorporating modified oligonucleotides. The sensors for our arrays were selectedto maximize their differential responses to the two steroids most different in an arbitrarily chosen parameternamed "shape-length". The arrays incorporated three previously unreported types of sensors identifiedthrough a massive screening effort: (1) three-way junction sensors with neutralized charges within junction;(2) "self-aggregating sensors"; and (3) sensors incorporating fluorophore directly in a three-way junctionas a spacer. The arrays were tested on seven steroids and an alkaloid (cocaine) over a range ofconcentrations, and achieved 92-96% accuracy in class assignments, despite the close structural similaritiesbetween steroids.