A Chemoselective Method for Site-Specific Immobilization of Peptides via Aminooxy Group
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- 作者：Maciej Adamczyk ; John C. Gebler ; Rajarathnam E. Reddy ; and Zhiguang Yu
- 刊名：Bioconjugate Chemistry
- 出版年：2001
- 出版时间：March 2001
- 年：2001
- 卷：12
- 期：2
- 页码：139 - 142
- 全文大小：63K
- 年卷期：v.12,no.2(March 2001)
- ISSN：1520-4812
文摘
Site-specific modification of peptides and proteins is an important area of basic research for preparationof well-defined biosensors and probes. The unique properties of aminooxy group present an opportunityfor chemoselective site-specific immobilization of peptides to prepare well-defined biosensors. We haveprepared FLAG peptide derivatives containing L-
-aminooxylysine (L--AOLys, 1a) and L-lysine unitsin their sequence at the C- and N-terminals via solid-phase synthesis. Site-specific modification ofpeptides through aminooxy group was demonstrated in the preparation of biosensors and selectiveconjugation in the preparation of biotinylated probes. Effect of the incorporation of L--AOLys (1a)into the peptide sequence and its subsequent labeling on the FLAG epitopic character was measuredusing a surface plasmon resonance detector. It was found that incorporation of L--AOLys (1a) intothe FLAG peptide and site-specific immobilization through aminooxy group preserved the integrityof FLAG epitope.
-aminooxylysine (L--AOLys, 1a) and L-lysine unitsin their sequence at the C- and N-terminals via solid-phase synthesis. Site-specific modification ofpeptides through aminooxy group was demonstrated in the preparation of biosensors and selectiveconjugation in the preparation of biotinylated probes. Effect of the incorporation of L--AOLys (1a)into the peptide sequence and its subsequent labeling on the FLAG epitopic character was measuredusing a surface plasmon resonance detector. It was found that incorporation of L--AOLys (1a) intothe FLAG peptide and site-specific immobilization through aminooxy group preserved the integrityof FLAG epitope.