Fluid and Resistive Tethered Lipid Membranes on Nanoporous Substrates

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• 作者：Gautam Gupta ; Kyle Staggs ; Aditya D. Mohite ; Jon K. Baldwin ; Srinivas Iyer ; Rangachary Mukundan ; Amit Misra ; Antonia Antoniou ; Andrew M. Dattelbaum
• 刊名：Journal of Physical Chemistry B
• 出版年：2015
• 出版时间：October 8, 2015
• 年：2015
• 卷：119
• 期：40
• 页码：12868-12876
• 全文大小：489K
• ISSN：1520-5207

文摘

Cell membranes perform important biological roles including compartmentalization, signaling, and transport of nutrients. Supported lipid membranes mimic the behavior of cell membranes and are an important model tool for studying membrane properties in a controlled laboratory environment. Lipid membranes may be supported on solid substrates; however, protein and lipid interactions with the substrate typically result in their denaturation. In this report, we demonstrate the formation of intact lipid membranes tethered on nanoporous metal thin films obtained via a dealloying process. Uniform lipid membranes were formed when the surface defect density of the nanoporous metal film was significantly reduced through a two-step dealloying process reported here. We show that the tethered lipid membranes on nanoporous metal substrates maintain both fluidity and electrical resistivity, which are key attributes to naturally occurring lipid membranes. The lipid assemblies supported on nanoporous metals provide a new platform for investigating lipid membrane properties, and potentially membrane proteins, for numerous applications including next generation biosensor platforms, targeted drug-delivery, and energy harvesting devices.