Characterization and Content of Flavonoid Glycosides in Genetically Modified Tomato (Lycopersicon esculentum) Fruits

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• 作者：Gwé ; na&euml ; lle Le Gall ; M. Susan DuPont ; Fred A. Mellon ; Adrienne L. Davis ; Geoff J. Collins ; Martine E. Verhoeyen ; and Ian J. Colquhoun
• 刊名：Journal of Agricultural and Food Chemistry
• 出版年：2003
• 出版时间：April 23, 2003
• 年：2003
• 卷：51
• 期：9
• 页码：2438 - 2446
• 全文大小：175K
• 年卷期：v.51,no.9(April 23, 2003)
• ISSN：1520-5118

文摘

There is a growing interest in producing food plants with increased amounts of flavonoids becauseof their potential health benefits. Tomatoes contain small amounts of flavonoids, most of which arelocated in the peel of the fruit. It has been shown that flavonoid accumulation in tomato flesh, andhence an overall increase in flavonoid levels in tomato fruit, can be achieved by means of simultaneousoverexpression of the maize transcription factors LC and C1. Fruit from progeny of two modifiedlines (2027 and 2059) was selected for a detailed analysis and individual identification of flavonoids,at different stages of maturity. Nine major flavonoids were detected in the flesh of transgenic ripetomatoes. LC/NMR, LC/MS, and LC/MS/MS enabled us to identify these as kaempferol-3,7-di-O-glucoside (1), kaempferol-3-O-rutinoside-7-O-glucoside (2), two dihydrokaempferol-O-hexosides (3and 4), rutin (5), kaempferol-3-O-rutinoside (6), kaempferol-3-O-glucoside (7), naringenin-7-O-glucoside (8) and naringenin chalcone (9), which were quantified by HPLC/DAD. All but 5, 6, and 9were detected in tomato for the first time. The total flavonoid glycoside content of ripe transgenictomatoes of line 2059 was about 10-fold higher than that of the controls, and kaempferol glycosidesaccounted for 60% of this. Kaempferol glycosides comprised around 5% of the flavonoid glycosidecontent of ripe control tomatoes (the rest was rutin and naringenin chalcone). The rutin concentrationin both transgenic and control fruits was similar.Keywords: Metabolomics; tomato; ripening; transgenic; GMO; flavonoids; kaempferol glycosides;naringenin-7-O-glucoside; dihydrokaempferol glycosides; transcription factors; LC; C1; Lycopersiconesculentum