Real Time, High Resolution Video Imaging of Apoptosis in Single Cells with a Polymeric Nanoprobe
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- 作者：Seulki Lee ; Ki Young Choi ; Hyunjin Chung ; Ju Hee Ryu ; Aeju Lee ; Heebeom Koo ; In-Chan Youn ; Jae Hyung Park ; In-San Kim ; Sang Yoon Kim ; Xiaoyuan Chen ; Seo Young Jeong ; Ick Chan Kwon ; Kwangmeyung Kim ; Kuiwon Choi
- 刊名：Bioconjugate Chemistry
- 出版年：2011
- 出版时间：February 16, 2011
- 年：2011
- 卷：22
- 期：2
- 页码：125-131
- 全文大小：971K
- 年卷期：v.22,no.2(February 16, 2011)
- ISSN：1520-4812
文摘
We report a new apoptosis nanoprobe (Apo-NP) designed on the basis of a polymer nanoparticle platform. This simple one-step technique is capable of boosting fluorescence signals upon apoptosis in living cells, enabling real-time imaging of apoptosis in single cells and in vivo. The Apo-NP efficiently delivers chemically labeled, dual-quenched caspase-3-sensitive fluorogenic peptides into cells, allowing caspase-3-dependent strong fluorescence amplification to be imaged in apoptotic cells in real-time and at high resolution. The design platform of the Apo-NP is flexible and can be fine-tuned for a wide array of applications such as identification of caspase-related apoptosis in pathologies and for monitoring therapeutic efficacy of apoptotic drugs in cancer treatment.