Quantitation of Transgenic Plant DNA in Leachate Water: Real-Time Polymerase Chain Reaction Analysis
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- 作者：Robert H. Gulden ; Sylvain Lerat ; Miranda M. Hart ; Jeff R. Powell ; Jack T. Trevors ; K. Peter Pauls ; John N. Klironomos ; and Clarence J. Swanton
- 刊名：Journal of Agricultural and Food Chemistry
- 出版年：2005
- 出版时间：July 27, 2005
- 年：2005
- 卷：53
- 期：15
- 页码：5858 - 5865
- 全文大小：233K
- 年卷期：v.53,no.15(July 27, 2005)
- ISSN：1520-5118
文摘
Roundup Ready (RR) genetically modified (GM) corn and soybean comprise a large portion of theannual planted acreage of GM crops. Plant growth and subsequent plant decomposition introducethe recombinant DNA (rDNA) into the soil environment, where its fate has not been completelyresearched. Little is known of the temporal and spatial distribution of plant-derived rDNA in the soilenvironment and in situ transport of plant DNA by leachate water has not been studied before. Theobjectives of this study were to determine whether sufficient quantities of plant rDNA were releasedby roots during growth and early decomposition to be detected in water collected after percolatingthrough a soil profile and to determine the influence of temperature on DNA persistence in the leachatewater. Individual plants of RR corn and RR soybean were grown in modified cylinders in a growthroom, and the cylinders were flushed with rain water weekly. Immediately after collection, the leachatewas subjected to DNA purification followed by rDNA quantification using real-time Polymerase ChainReaction (PCR) analysis. To test the effects of temperature on plant DNA persistence in leachatewater, water samples were spiked with known quantities of RR soybean or RR corn genomic DNAand DNA persistence was examined at 5, 15, and 25 
C. Differences in the amounts and temporaldistributions of root-derived rDNA were observed between corn and soybean plants. The resultssuggest that rainfall events may distribute plant DNA throughout the soil and into leachate water.Half-lives of plant DNA in leachate water ranged from 1.2 to 26.7 h, and persistence was greater atcolder temperatures (5 and 15 C).Keywords: Agriculture; Biotechnology; corn; DNA half-life; environment; genetically modified;glyphosate; leachate; method; real-time PCR; roots; soil; soil columns; soybean; temperature; transgenicplants; water
C. Differences in the amounts and temporaldistributions of root-derived rDNA were observed between corn and soybean plants. The resultssuggest that rainfall events may distribute plant DNA throughout the soil and into leachate water.Half-lives of plant DNA in leachate water ranged from 1.2 to 26.7 h, and persistence was greater atcolder temperatures (5 and 15 C).Keywords: Agriculture; Biotechnology; corn; DNA half-life; environment; genetically modified;glyphosate; leachate; method; real-time PCR; roots; soil; soil columns; soybean; temperature; transgenicplants; water