Revisiting 尾-Casein as a Stabilizer for Lipid Liquid Crystalline Nanostructured Particles

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• 作者：Jiali Zhai ; Lynne Waddington ; Tim J. Wooster ; Marie-Isabel Aguilar ; Ben J. Boyd
• 刊名：Langmuir
• 出版年：2011
• 出版时间：December 20, 2011
• 年：2011
• 卷：27
• 期：24
• 页码：14757-14766
• 全文大小：1125K
• 年卷期：v.27,no.24(December 20, 2011)
• ISSN：1520-5827

文摘

Lipid liquid crystalline nanoparticles such as cubosomes and hexosomes have unique internal nanostructures that have shown great potential in drug and nutrient delivery applications. The triblock copolymer, Pluronic F127, is usually employed as a steric stabilizer in dispersions of lipid nanostructured particles. In this study, we investigated the formation, colloidal stability and internal nanostructure and morphology of glyceryl monooleate (GMO) and phytantriol (PHYT) cubosome dispersions on substituting 尾-casein with F127 in increasing proportion as the stabilizer. Internal structure and particle morphology were evaluated using small-angle X-ray scattering (SAXS) and cryo-transmission electron microscopy (cryo-TEM), while protein secondary structure was studied using synchrotron radiation circular dichroism (SRCD). The GMO cubosome dispersion stabilized by 尾-casein alone displayed a V₂ (Pn3m) phase structure and a V₂ to H₂ phase transition at 60 掳C. In comparison, F127-stabilized GMO dispersion had a V₂ (Im3m) phase structure and the H₂ phase only appeared at higher temperature, that is, 70 掳C. In the case of PHYT dispersions, only the V₂ (Pn3m) phase structure was observed irrespective of the type and concentration of stabilizers. However, 尾-casein-stabilized PHYT dispersion displayed a V₂ to H₂ to L₂ transition behavior upon heating, whereas F127-stabilized PHYT dispersion displayed only a direct V₂ to L₂ transition. The protein secondary structure was not disturbed by interaction with GMO or PHYT cubosomes. The results demonstrate that 尾-casein provides steric stabilization to dispersions of lipid nanostructured particles and avoids the transition to Im3m structure in GMO cubosomes, but also favors the formation of the H₂ phase, which has implications in drug formulation and delivery applications.