Characterization of Lipid Fatty Acids in Whole-Cell Microorganisms Using in Situ Supercritical Fluid Derivatization/Extraction and Gas Chromatography/Mass Spectrometry
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- 作者：Ahmad A. Gharaibeh and Kent J. Voorhees
- 刊名：Analytical Chemistry
- 出版年：1996
- 出版时间：September 1, 1996
- 年：1996
- 卷：68
- 期：17
- 页码：2805 - 2810
- 全文大小：484K
- 年卷期：v.68,no.17(September 1, 1996)
- ISSN：1520-6882
文摘
In situ supercritical fluid derivatization and extractionwasused as a sample preparation technique for the classification of bacteria using fatty acid profiling. Addition ofaquaternary ammonium salt such as phenyltrimethylammonium hydroxide under static supercritical conditionsdirectly to lyophilized, whole-cell bacteria in anextractionvessel resulted in the saponification of the bacteriallipidsand derivatization of their fatty acids. The derivatizedfattyacid methyl esters (FAMEs) were then extracted withsupercritical CO2 and analyzed without additionaltreatment using GC/MS. Iso and anteiso C15:0 and C17:0along with C18:0 were predominant in Gram-positivebacteria, while C16:1, C16:0, C18:1, and cyclopropylcyC17:0 and cyC19:0 were significant in Gram-negativebacteria. Application of principal components analysistothe FAME GC/MS data resulted in the differentiationbetween Gram-positive and Gram-negative type bacteria.Differentiation between species among the same generawas also observed.