Applicability of the Quantification of Genetically Modified Organisms to Foods Processed from Maize and Soy

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• 作者：Tomoaki Yoshimura ; Hideo Kuribara ; Takeshi Matsuoka ; Takashi Kodama ; Mayu Iida ; Takahiro Watanabe ; Hiroshi Akiyama ; Tamio Maitani ; Satoshi Furui ; and Akihiro Hino
• 刊名：Journal of Agricultural and Food Chemistry
• 出版年：2005
• 出版时间：March 23, 2005
• 年：2005
• 卷：53
• 期：6
• 页码：2052 - 2059
• 全文大小：1594K
• 年卷期：v.53,no.6(March 23, 2005)
• ISSN：1520-5118

文摘

The applicability of quantifying genetically modified (GM) maize and soy to processed foods wasinvestigated using heat treatment processing models. The detection methods were based on real-time quantitative polymerase chain reaction (PCR) analysis. Ground seeds of insect resistant GMmaize (MON810) and glyphosate tolerant Roundup Ready (RR) soy were dissolved in water andwere heat treated by autoclaving for various time intervals. The calculated copy numbers of therecombinant and taxon specific deoxyribonucleic acid (DNA) sequences in the extracted DNA solutionwere found to decrease with time. This decrease was influenced by the PCR-amplified size. Theconversion factor (C_f), which is the ratio of the recombinant DNA sequence to the taxon specificDNA sequence and is used as a constant number for calculating GM% at each event, tended to bestable when the sizes of PCR products of two DNA sequences were nearly equal. The resultssuggested that the size of the PCR product plays a key role in the quantification of GM organisms inprocessed foods. It is believed that the C_f of the endosperm (3n) is influenced by whether the GMoriginated from a paternal or maternal source. The embryos and endosperms were separated fromthe F1 generation seeds of five GM maize events, and their C_f values were measured. Both paternaland maternal GM events were identified. In these, the endosperm C_f was lower than that of theembryo, and the embryo C_f was lower than that of the endosperm. These results demonstrate thedifficulties encountered in the determination of GM% in maize grains (F2 generation) and in processedfoods from maize and soy.Keywords: Zea mays; Glycine max; genetically modified; MON810; Roundup Ready; processed food;endosperm; embryo; heat treatment; GMO detection; quantitative analysis