Reduction of Antigenicity of Cry j 1, a Major Allergen of Japanese Cedar Pollen, by Thermal Denaturation
详细信息 查看全文
- 作者：Rieko Aoki ; Akira Saito ; Masakatsu Usui ; Hiroyuki Azakami ; Akio Kato
- 刊名：Journal of Agricultural and Food Chemistry
- 出版年：2009
- 出版时间：June 10, 2009
- 年：2009
- 卷：57
- 期：11
- 页码：4995-4999
- 全文大小：711K
- 年卷期：v.57,no.11(June 10, 2009)
- ISSN：1520-5118
文摘
The soluble aggregates of Cry j 1, a major allergen of Japanese cedar pollen, were formed without any coagulates during heat treatment at acidic pH 5, as shown in HPLC and SDS−PAGE patterns. A remarkable change in the CD spectrum was observed between native and heat-denatured Cry j 1 at a linear rate of 1 °C/min from 40 to 90 °C. The negative peak of native Cry j 1 at 222 nm was moved to 218 nm, suggesting the transition of an α-helix to β-structure during heat denaturation. The increase in β-structure was also observed during heat denaturation by monitoring the fluorescence with Thioflavin T. These results suggest that Cry j 1 forms intermolecular cross-β-structure between denatured proteins during heating at 90 °C. The antigenicity of Cry j 1 detected by dot-blotting was greatly diminished during heating at a linear rate of 1 °C/min from 40 to 90 °C without any coagulates. These results suggest that IgE epitopes exposed on the molecular surface of Cry j 1 was buried inside soluble aggregates through intermolecular β-structure formed by heating.