Effects of Various Saccharides on the Masking of Epitope Sites and Uptake in the Gut of Cedar Allergen Cry j 1−Saccharide Conjugates by a Naturally Occurring Maillard Reaction

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• 作者：Reiko Aoki ; Akira Saito ; Hiroyuki Azakami ; Akio Kato
• 刊名：Journal of Agricultural and Food Chemistry
• 出版年：2010
• 出版时间：July 14, 2010
• 年：2010
• 卷：58
• 期：13
• 页码：7986-7990
• 全文大小：754K
• 年卷期：v.58,no.13(July 14, 2010)
• ISSN：1520-5118

文摘

A major allergen of Japanese cedar, Cry j 1, was conjugated with galactomannan (M_w of 15 kDa), dextran (M_w of 12 kDa), xyloglucan (M_w of 1.4 kDa), and various monosaccharides through the Maillard reaction by dry-heating in 65% relative humidity. The Cry j 1−galactomannan conjugate completely masked the epitopes of the allergen in Cry j 1. The Cry j 1−dextran conjugate also masked the epitopes of Cry j 1. The small size of oligosaccharide (xyloglucan) and various monosaccharides cannot mask the epitopes of allergen Cry j 1. This suggests that the higher molecular size of attached saccharides is important to mask sterically the epitope sites. The Cry j 1−galactomannan and Cry j 1−mannose conjugates were effectively trafficked in the gut and co-localized with immune cells, such as dendritic cells in the gut, suggesting that Cry j 1−saccharide conjugates are phagocytosed via the mannose receptor in immune cells. These results suggest that the Cry j 1−galactomannan conjugate is suitable for masking the epitope sites of Cry j 1 and trafficking to immune cells in gut lumen.

Keywords:

Japanese cedar pollen allergen Cry j 1; allergen Cry j 1−saccharide conjugate; Maillard reaction; masking of epitope; immune cell; dendritic cell; mannose receptor; galactomannan