Fatty liver indu
ced by al
cohol abuse is a major worldwide health hazard leading to morbidity and mortality. Previous studies indi
cate antifatty liver properties of garli
c. This study investigated the mole
cular me
chanisms of garli
c oil (GO) or diallyl disulfide (DADS) imparted hepatoprote
ction against al
cohol indu
ced fatty liver in C57BL/6 mi
ce using mi
croarray-based global gene expression analysis. Al
cohol liquid diet resulted in severe fatty liver with in
creased levels of serum aspartate aminotransferease and
alanine aminotransferease as well as trigly
cerides and de
creased levels of liver glutathione and antioxidant enzymes. The major
canoni
cal pathways impli
cated by al
cohol treatment are the metabolisms of xenobioti
cs by
cyto
chrome P450, glutathione, and ara
chidoni
c a
cid. Treatment with DADS or GO normalized the serum aminotransferease levels and liver antioxidant enzymes and redu
ced the
contents of trigly
cerides and
cholesterol. The
canoni
cal pathways involved in the amelioration of liver in
clude ara
chidoni
c a
cid metabolism, altered T
cell and B
cell signaling, tryptophan metabolism, antigen presentation pathway for DADS, metabolism of xenobioti
cs, mitoti
c roles of Polo-like kinase, fatty a
cid metabolism, LPS/IL-1 mediated inhibition of RXR fun
ction, and C21-steroid hormone metabolism for GO.
Keywords:
cs.org/action/doSearch?action=search&searchText=antifatty+liver&qsSearchArea=searchText">antifatty liver; cs.org/action/doSearch?action=search&searchText=diallyl+disulfide&qsSearchArea=searchText">diallyl disulfide; cs.org/action/doSearch?action=search&searchText=garlic+oil&qsSearchArea=searchText">garlic oil; cs.org/action/doSearch?action=search&searchText=mechanism&qsSearchArea=searchText">mechanism; cs.org/action/doSearch?action=search&searchText=transcriptome&qsSearchArea=searchText">transcriptome