Brominated Lipids Identify Lipid Binding Sites on the Surface of the Reaction Center from Rhodobacter sphaeroides
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- 作者：Aleksander W. Roszak ; Alastair T. Gardiner ; Neil W. Isaacs ; Richard J. Cogdell
- 刊名：Biochemistry
- 出版年：2007
- 出版时间：March 20, 2007
- 年：2007
- 卷：46
- 期：11
- 页码：2909 - 2916
- 全文大小：1146K
- 年卷期：v.46,no.11(March 20, 2007)
- ISSN：1520-4995
文摘
This study describes the use of brominated phospholipids to distinguish between lipid anddetergent binding sites on the surface of a typical 
-helical membrane protein. Reaction centers isolatedfrom Rhodobacter sphaeroides were cocrystallized with added brominated phospholipids. X-ray structuralanalysis of these crystals has revealed the presence of two lipid binding sites from the characteristicstrong X-ray scattering from the bromine atoms. These results demonstrate the usefulness of this approachto mapping lipid binding sites at the surface of membrane proteins.
-helical membrane protein. Reaction centers isolatedfrom Rhodobacter sphaeroides were cocrystallized with added brominated phospholipids. X-ray structuralanalysis of these crystals has revealed the presence of two lipid binding sites from the characteristicstrong X-ray scattering from the bromine atoms. These results demonstrate the usefulness of this approachto mapping lipid binding sites at the surface of membrane proteins.