Construction and Structural Analysis of Tethered Lipid Bilayer Containing Photosynthetic Antenna Proteins for Functional Analysis
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- 作者：Ayumi Sumino ; Takehisa Dewa ; Toshikazu Takeuchi ; Ryuta Sugiura ; Nobuaki Sasaki ; Nobuo Misawa ; Ryugo Tero ; Tsuneo Urisu ; Alastair T. Gardiner ; Richard J. Cogdell ; Hideki Hashimoto ; Mamoru Nango
- 刊名：Biomacromolecules
- 出版年：2011
- 出版时间：July 11, 2011
- 年：2011
- 卷：12
- 期：7
- 页码：2850-2858
- 全文大小：1089K
- 年卷期：v.12,no.7(July 11, 2011)
- ISSN：1526-4602
文摘
The construction and structural analysis of a tethered planar lipid bilayer containing bacterial photosynthetic membrane proteins, light-harvesting complex 2 (LH2), and light-harvesting core complex (LH1-RC) is described and establishes this system as an experimental platform for their functional analysis. The planar lipid bilayer containing LH2 and/or LH1-RC complexes was successfully formed on an avidin-immobilized coverglass via an avidin鈥揵iotin linkage. Atomic force microscopy (AFM) showed that a smooth continuous membrane was formed there. Lateral diffusion of these membrane proteins, observed by a fluorescence recovery after photobleaching (FRAP), is discussed in terms of the membrane architecture. Energy transfer from LH2 to LH1-RC within the tethered membrane was observed by steady-state fluorescence spectroscopy, indicating that the tethered membrane can mimic the natural situation.